Clusterin facilitates in vivo clearance of extracellular misfolded proteins
Identifieur interne : 001571 ( Main/Exploration ); précédent : 001570; suivant : 001572Clusterin facilitates in vivo clearance of extracellular misfolded proteins
Auteurs : Amy R. Wyatt [Australie] ; Justin J. Yerbury [Australie] ; Paula Berghofer [Australie] ; Ivan Greguric [Australie] ; Andrew Katsifis [Australie] ; Christopher M. Dobson [Royaume-Uni] ; Mark R. Wilson [Australie]Source :
- Cellular and Molecular Life Sciences [ 1420-682X ] ; 2011-12-01.
English descriptors
- KwdEn :
Abstract
Abstract: The extracellular deposition of misfolded proteins is a characteristic of many debilitating age-related disorders. However, little is known about the specific mechanisms that act to suppress this process in vivo. Clusterin (CLU) is an extracellular chaperone that forms stable and soluble complexes with misfolded client proteins. Here we explore the fate of complexes formed between CLU and misfolded proteins both in vitro and in a living organism. We show that proteins injected into rats are cleared more rapidly from circulation when complexed with CLU as a result of their more efficient localization to the liver and that this clearance is delayed by pre-injection with the scavenger receptor inhibitor fucoidan. The CLU–client complexes were found to bind preferentially, in a fucoidan-inhibitable manner, to human peripheral blood monocytes and isolated rat hepatocytes and in the latter cell type were internalized and targeted to lysosomes for degradation. The data suggest, therefore, that CLU plays a key role in an extracellular proteostasis system that recognizes, keeps soluble, and then rapidly mediates the disposal of misfolded proteins.
Url:
DOI: 10.1007/s00018-011-0684-8
Affiliations:
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Life Sci.</title><idno type="ISSN">1420-682X</idno><idno type="eISSN">1420-9071</idno><imprint><publisher>SP Birkhäuser Verlag Basel</publisher><pubPlace>Basel</pubPlace><date type="published" when="2011-12-01">2011-12-01</date><biblScope unit="volume">68</biblScope><biblScope unit="issue">23</biblScope><biblScope unit="page" from="3919">3919</biblScope><biblScope unit="page" to="3931">3931</biblScope></imprint><idno type="ISSN">1420-682X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1420-682X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Clearance</term><term>Clusterin</term><term>Extracellular chaperone</term><term>Misfolded protein</term><term>Receptor-mediated endocytosis</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The extracellular deposition of misfolded proteins is a characteristic of many debilitating age-related disorders. 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Wyatt</name></noRegion><name sortKey="Berghofer, Paula" sort="Berghofer, Paula" uniqKey="Berghofer P" first="Paula" last="Berghofer">Paula Berghofer</name><name sortKey="Greguric, Ivan" sort="Greguric, Ivan" uniqKey="Greguric I" first="Ivan" last="Greguric">Ivan Greguric</name><name sortKey="Katsifis, Andrew" sort="Katsifis, Andrew" uniqKey="Katsifis A" first="Andrew" last="Katsifis">Andrew Katsifis</name><name sortKey="Wilson, Mark R" sort="Wilson, Mark R" uniqKey="Wilson M" first="Mark R." last="Wilson">Mark R. Wilson</name><name sortKey="Wilson, Mark R" sort="Wilson, Mark R" uniqKey="Wilson M" first="Mark R." last="Wilson">Mark R. Wilson</name><name sortKey="Wilson, Mark R" sort="Wilson, Mark R" uniqKey="Wilson M" first="Mark R." last="Wilson">Mark R. Wilson</name><name sortKey="Wyatt, Amy R" sort="Wyatt, Amy R" uniqKey="Wyatt A" first="Amy R." last="Wyatt">Amy R. Wyatt</name><name sortKey="Yerbury, Justin J" sort="Yerbury, Justin J" uniqKey="Yerbury J" first="Justin J." last="Yerbury">Justin J. Yerbury</name><name sortKey="Yerbury, Justin J" sort="Yerbury, Justin J" uniqKey="Yerbury J" first="Justin J." last="Yerbury">Justin J. Yerbury</name></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Dobson, Christopher M" sort="Dobson, Christopher M" uniqKey="Dobson C" first="Christopher M." last="Dobson">Christopher M. Dobson</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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